经颅磁刺激对老年大鼠工作记忆相关跨脑区网络协同作用的影响

郭苗苗; 翟昊迪; 吉利辉; 王田; 徐桂芝

doi:10.11999/JEIT230291

经颅磁刺激对老年大鼠工作记忆相关跨脑区网络协同作用的影响

doi: 10.11999/JEIT230291

郭苗苗^{1, 2, 3, 4, ,},
翟昊迪^{1, 2, 3, 4},
吉利辉^{1, 2, 3, 4},
王田^{1, 2, 3, 4},
徐桂芝^{1, 2, 3}

1.
省部共建电工装备可靠性与智能化国家重点实验室(河北工业大学) 天津 300130
2.
河北省生物电磁与神经工程重点实验室(河北工业大学) 天津 300130
3.
天津市生物电工与智能健康重点实验室(河北工业大学) 天津 300130
4.
河北工业大学生命科学与健康工程学院天津 300130

基金项目: 国家自然科学基金(51737003, 51707054)，国家重点研发计划(2022YFC2402203)，国家自然科学基金国际合作与交流项目(523201050)，河北省自然科学基金(E2021202222)，河北省基础研究计划(18963001D)，省部共建电工装备可靠性与智能化国家重点实验室(河北工业大学)优秀青年创新基金(EERI_OY2021009)，河北省省级科技计划资助(225676163GH)

详细信息

作者简介:
郭苗苗：女，博士，副教授，研究方向为脑认知与神经工程

翟昊迪：男，硕士生，研究方向为生物电磁与神经调控

吉利辉：男，硕士生，研究方向为认知与神经工程

王田：女，博士生，研究方向为生物电磁与神经调控

徐桂芝：女，博士，教授，研究方向为生物电磁技术

通讯作者:
郭苗苗　gmm@hebut.edu.cn

中图分类号: TN911.7; R318; R338
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- 被引次数: 0
出版历程
- 收稿日期: 2023-04-18
- 网络出版日期: 2024-03-13
- 刊出日期: 2024-04-24

Effect of Transcranial Magnetic Stimulation on Inter-brain Region Networks of Aged Rats during Working Memory Task

GUO Miaomiao^{1, 2, 3, 4
, ,},
ZHAI Haodi^{1, 2, 3, 4},
JI Lihui^{1, 2, 3, 4},
WANG Tian^{1, 2, 3, 4},
XU Guizhi^{1, 2, 3}

1.
State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, China
2.
Hebei Key Laboratory of Bioelectromagnetics and Neural Engineering, Hebei University of Technology, Tianjin 300130, China
3.
Tianjin Key Laboratory of Bioelectromagnetic Technology and Intelligent Health, Hebei University of Technology, Tianjin 300130, China
4.
School of Health Sciences & Biomedical Engineering, Hebei University of Technology, Tianjin 300130, China

Funds: The National Natural Science Foundation of China (51737003, 51707054), The National Key Research and Development Program project (2022YFC2402203), The National Natural Science Foundation International Cooperation and exchange project (523201050), The Natural Science Foundation of Hebei Province (E2021202222), The Basic Research Program of Hebei Province (18963001D), The Excellent Youth Innovation Fund of the State Key Laboratory of Reliability and Intelligence of Electrical Equipment (Hebei University of Technology) (EERI_OY2021009), S&T Program of Hebei (225676163GH)

摘要

摘要: 经颅磁刺激(TMS)因其可以非侵入性地探测和调制大脑皮层兴奋性和功能，已被广泛地应用于改善大脑认知功能等临床神经调控领域。工作记忆的功能实现需要多个脑区的同步活动，该文将行为学与电生理学相结合，通过建立跨脑区的因果网络连接，从记忆相关脑区间协同作用的角度探究不同模式的TMS对大脑认知功能的调控机制。首先对老年威斯塔大鼠分别实施重复经颅磁刺激(rTMS)及间歇性θ节律刺激(iTBS)，并设置空白对照组，通过在体多通道微电极阵列采集大鼠工作记忆任务中的局部场电位信号(LFPs)；之后基于定向传递函数构建LFPs脑因果网络；最后通过对比行为学结果差异、各脑区因果网络参数等，探索不同模式TMS对老年大鼠工作记忆行为学及脑区之间信息协同作用的影响规律。结果表明，rTMS组和iTBS组大鼠执行正确工作记忆任务的平均天数减少，平均正确率高于空白对照组。经过刺激后，rTMS组和iTBS组前额叶和海马双向网络连接明显增强，信息流强度和因果流向性得到显著提升(P＜0.05)。因此，rTMS和iTBS模式均能促进老年大鼠海马和前额叶脑区间的信息交流，从而使老年大鼠的工作记忆能力得到改善。
- 重复经颅磁刺激 /
- 间歇性${\text{θ}} $节律刺激 /
- 工作记忆 /
- 脑因果网络
Abstract: Transcranial Magnetic Stimulation (TMS) has been widely used in clinical neuroregulation fields such as improving brain cognitive function because of its ability to non-invasibly detect and modulate the excitability and function of the cerebral cortex. The functional realization of working memory requires the synchronous activity of multiple brain regions. In this paper, it is of great significance to combine behavioral and electrophysiology, establish causal network connections across brain regions, and explore the regulatory mechanism of different modes of TMS on brain cognitive function from the perspective of memory-related brain regions. Firstly, repetitive Transcranial Magnetic Stimulation (rTMS) and intermittent Theta Burst Stimulation (iTBS) are performed in aged Wistar rats, a blank control group is established at the same time, and the Local Field Potentials (LFPs) are collected by an in vivo multichannel microelectrode array during a working memory task. Then, the LFPs brain causal network is constructed based on the directed transfer function. Finally, by comparing the behavioral results and the causal network parameters of each brain region, the effects of TMS on the working memory behavior and the information synergy between brain regions are explored. The results show that the average number of days that rats in the rTMS and iTBS groups performed the correct working memory task is reduced, and the average correct rate is higher than that in the blank control group. After stimulation, the bidirectional network connectivity between prefrontal lobe and hippocampus in the rTMS group and the iTBS group is significantly enhanced, and the strength of information flow and causal streamability are significantly improved (P < 0.05). Therefore, both rTMS and iTBS can promote the communication between the hippocampus and prefrontal cortex, thereby improving the working memory ability of aged rats.
- Repetitive Transcranial Magnetic Stimulation (rTMS) /
- Intermittent Theta Burst Stimulation(iTBS) /
- Working memory /
- Brain causal network

HTML全文

图 1 两种TMS模式示意图

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图 2 T迷宫任务

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图 3 4组大鼠的行为学正确率及学会天数

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图 4 4组大鼠${\text{θ}} $频段和${\text{γ}} $频段前额叶和海马双向网络

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图 5 4组大鼠跨脑区${\text{θ}} $频段和${\text{γ}} $频段的连接密度和全局效率

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图 6 四组大鼠${\text{θ}} $频段和${\text{γ}} $频段前额叶和海马双向信息流随时间变化曲线

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图 7 4组大鼠${\text{θ}} $频段和${\text{γ}} $频段前额叶和海马双向信息流峰值

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图 8 4组大鼠各频段因果流向及平均因果流向值

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