Abstract: Direct Sequence Spread Spectrum (DSSS) provides an effective way for underwater acoustic communication under low Signal-to-Noise Ratio (SNR). However, low SNR will deteriorate the common multi-path mitigation approaches employed in DSSS system such as channel equalizer and rake receiver. Considering that multi-channel time reversal has the ability to refocus the energy in spatial and temporal domain, multi-channel time reversal is incorporated with DSSS technology to suppress the multipath interference under low SNR, also convolutional coding is adopted to further improve the communication performance. Lake trial results show the effectiveness of the proposed scheme.