Abstract: The Received Signal Strength Indicator (RSSI) based location is easy to be affected by multipath interference in wireless sensor networks. The traditional RSSI-based location methods usually increase the number of samples to resist multipath interference while few works are done on physical layer. This paper studies to separate subchip multipath components for RSSI-based location application, the direct path can be separated among multipath by despreaded using the first sample of each chip, after calculating the direct paths power the anti-multipath interferences RSSI value can be got. The theoretical analysis of the performance of separating subchip multipath, Signal-to-Noise Ratio (SNR) losses are presented. Finally, the simulation shows the variance of RSSI value calculated by the traditional method and the proposal in this paper, the variance of the proposed method is smaller than the traditional method under multipath channel environment.