光电镊—一种用途广泛的微纳操作工具

张帅龙; 李恭; 李凤刚; 徐冰睿; 李航; 符荣鑫

doi:10.11999/JEIT221315

光电镊—一种用途广泛的微纳操作工具

doi: 10.11999/JEIT221315

张帅龙^{1, 2, 3, 4},
李恭^{1, 2},
李凤刚^{1, 2},
徐冰睿^{1, 2},
李航^{3, 5},
符荣鑫^{3, 5, ,}

1.
北京理工大学机电学院北京 100081
2.
北京市智能机器人与系统高精尖创新中心北京 100081
3.
北京理工大学医工融合研究院北京 100081
4.
北京理工大学仿生机器人与系统教育部重点实验室北京 100081
5.
北京理工大学医学技术学院北京 100081

基金项目: 国家自然科学基金(62103050, 62105177, 21904008)，国家海外高层次人才计划青年项目

详细信息

作者简介:
张帅龙：男，博士，教授，博士生导师，主要研究方向为生物微纳操作系统、光电镊技术、数字微流控技术、生物检测

李恭：男，硕士生，研究方向为基于光电镊技术的微操控

李凤刚：男，博士生，主要研究方向为基于光电镊技术的微操控及数字微流控

徐冰睿：女，博士生，主要研究方向为光操控技术与微生物行为学

李航：女，博士，副研究员，硕士生导师，主要研究方向为质谱分析与蛋白质组学

符荣鑫：男，博士，副研究员，硕士生导师，主要研究方向为光谱计算与微流控生物传感器

通讯作者:
符荣鑫　furongxin@bit.edu.cn

中图分类号: TP212; O436
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- 被引次数: 0
出版历程
- 收稿日期: 2022-10-19
- 修回日期: 2023-01-16
- 网络出版日期: 2023-02-22
- 刊出日期: 2023-12-26

Optoelectronic Tweezers — A Versatile Micro/Nano Operation Technique

ZHANG Shuailong^{1, 2, 3, 4},
LI Gong^{1, 2},
LI Fenggang^{1, 2},
XU Bingrui^{1, 2},
LI Hang^{3, 5},
FU Rongxin^{3, 5
, ,}

1.
School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China
2.
Beijing Advanced Innovation Center for Intelligent Robots and Systems, Beijing 100081, China
3.
Institute of Medical and Engineering Integration, Beijing Institute of Technology, Beijing 100081, China
4.
Key Laboratory of Biomimetic Robots and Systems of Ministry of Education, Beijing Institute of Technology, Beijing 100081, China
5.
School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China

Funds: The National Natural Science Foundation of China (62103050, 62105177, 21904008), The Recruitment Program of Global Experts and National Special Support Plan for High-level Talents

摘要

摘要: 光电镊(OET)是一种基于光致介电泳效应的微尺度光操控技术，可在流体场、光电场、生物力场耦合的复杂环境下对微小目标进行精准操控，在细胞操作、微机械系统等领域有着重要的应用。光电镊技术可以单独使用或与其他技术协同使用，应用十分广泛。迄今为止，基于光电镊的研究主要集中在：微纳米材料的操作、组装和合成；单个细胞/分子的操作、分离和分析；细胞固有特性的分析和获取；细胞的电穿孔、融合和裂解；细胞封装生物材料和生物结构的制备；流体传输的光流体器件的开发。这些工作展示了光电镊技术优越的性能和独特的通用性和灵活性。该文系统地介绍了光电镊技术的现有应用，总结了该技术的应用前景、局限性及发展趋势。
- 光操控 /
- 微机械 /
- 光电镊 /
- 微操作 /
- 介电泳
Abstract: OptoElectronic Tweezer (OET) is a micro-scale optical manipulation technology based on photoinduced electrophoretic effect. It can accurately control small targets in the complex environment of fluid field, photoelectric field and biological force field, and has important applications to cell operation, micromechanical system and other fields. Optoelectronic tweezers technology can be used alone or in conjunction with other technologies, and has been widely used. To date, research based on optoelectronic tweezers has focused on manipulation, assembly, and synthesis of micro and nanomaterials; manipulation, isolation, and analysis of individual cells/molecules; analysis and acquisition of cell intrinsic properties; electroporation, fusion, and lysis of cells; preparation of cell-encapsulated biomaterials and biological structures; development of optical fluid devices for fluid transport. These works demonstrate the superior performance and unique versatility and flexibility of the optoelectronic tweezers technology. The existing application of optoelectronic tweezers technology are systematically presented in the paper and the application prospect, limitation and development trend of this technology are summarised.
- Optical manipulation /
- Micromachine /
- Optoelectronic tweezers /
- Micromanipulation /
- Dielectrophoresis

HTML全文

图 1 3种光电镊设备^[10]

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图 2 使用微生物运输微小物体^[11]

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图 3 光诱导单细胞电穿孔^[12]

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图 4 细胞在接收点进行光诱导细胞融合^[13]

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图 5 细胞基于光电镊的1阶响应^[14]

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图 6 利用收缩光圈富集细胞外囊泡^[15]

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图 7 光电镊结合数字微流控技术^[16]

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图 8 Beacon光电镊平台^[17]

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图 9 基于光电镊技术印制微电路^[19]

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图 10 可进行并行操作的光电镊微机器人^[20]

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图 11 光电镊驱动微齿轮组实现物体的3维运输^[21]

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