单细胞结构和电学特征检测方法

梁红雁; 陈德勇; 王军波; 陈健

doi:10.11999/JEIT211459

单细胞结构和电学特征检测方法

doi: 10.11999/JEIT211459

梁红雁^{1, 2},
陈德勇^{1, 2, 3},
王军波^{1, 2, 3},
陈健^{1, 2, 3, ,}

1.
中国科学院空天信息创新研究院传感技术国家重点实验室北京 100190
2.
中国科学院大学电子电气与通信工程学院北京 100049
3.
中国科学院大学未来技术学院北京 100049

基金项目: 国家自然科学基金(61825107, 61922079, 62121003)

详细信息

作者简介:
梁红雁：女，1994年生，博士生，研究方向为单细胞检测分析

陈德勇：男，1967年生，研究员，研究方向为MEMS传感技术

王军波：男，1973年生，研究员，研究方向为MEMS传感技术

陈健：男，1981年生，研究员，研究方向为单细胞检测分析

通讯作者:
陈健　chenjian@mail.ie.ac.cn

中图分类号: TP212.3
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- 被引次数: 0
出版历程
- 收稿日期: 2021-12-08
- 修回日期: 2022-03-17
- 网络出版日期: 2022-04-16
- 刊出日期: 2022-08-17

Methods for Measuring Single-Cell Structural and Electrical Properties

LIANG Hongyan^{1, 2},
CHEN Deyong^{1, 2, 3},
WANG Junbo^{1, 2, 3},
CHEN Jian^{1, 2, 3
, ,}

1.
State Key Laboratory of Transducer Technology, Aerospace Information Research Institute of Chinese Academy of Sciences, Beijing 100190, China
2.
School of Electronic, Electrical and Communication Engineering of University of Chinese Academy of Sciences, Beijing 100049, China
3.
School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China

Funds: The National Natural Science Foundation of China (61825107, 61922079, 62121003)

摘要

摘要: 单细胞固有生物物理学特征，主要包括结构特征如细胞直径和细胞核直径，以及电学特征如细胞膜比电容和细胞质电导率，已经被应用于细胞亚类型分类和细胞状态评估，在生物医学研究和临床诊断方面具有广阔的应用前景。该文综述了不同类型的单细胞结构和电学特征检测方法，介绍了固定式、流动式以及基于微流控的方法。归纳总结了这些方法的工作原理、发展和主要优缺点，探讨了单细胞结构和电学特征检测所面临的挑战以及未来的研究机遇。
- 单细胞分析 /
- 结构特征 /
- 电学特征 /
- 微流控
Abstract: Single-cell intrinsic biophysical properties including structural properties (e.g., cellular diameter and nuclear diameter) and electrical properties (e.g., specific membrane capacitance and cytoplasmic conductivity) have been applied to cell subtype classifications, and cell status evaluations are promising in biomedical research and clinical diagnosis. Different types of single-cell structural and electrical properties detection methods are reviewed in this paper, then fixed and flow methods and microfluidic methods are introduced. The working principles, developments, main advantages and disadvantages of these methods are summarized, and the challenges and future research opportunities for measurements of single-cell structural and electrical properties are discussed.
- Single-cell analysis /
- Structural properties /
- Electrical properties /
- Microfluidics

HTML全文

图 1 固定式检测方法

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图 2 流动式检测方法

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图 3 基于微流控检测方法

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图 4 基于微流控检测方法(多电极)

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图 5 基于微流控检测方法(“压缩通道”)

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表 1 单细胞结构和电学特征检测方法标志性发展

方法		检测参数	区分目标细胞/关键成就	参考文献
固定式	膜片钳	细胞膜电容	表征数十个细胞电学特征	[30]
	电旋转	细胞膜比电容	表征数百个细胞电学特征	[47]
	介电电泳	细胞膜比电容和细胞质电导率	表征细胞群体平均电学特征	[55]
流动式	电阻抗	直流阻抗数据；直流+交流阻抗数据	白细胞3分群；白细胞5分类	[56]
	光散射	前向+侧向散射光；多角度散射光	白细胞3分群；白细胞5分类	[56]
	光成像	荧光成像	表征细胞直径和细胞核直径	[67]
微流控方法	共面电极	双频阻抗数据	5, 8 μm珠子；红细胞及其血影细胞	[68]
	对面电极	双频阻抗数据	5, 6 μm珠子；红细胞及其固定细胞	[69]
	对面电极+光学透镜	双频阻抗数据和荧光信号	白细胞3分群	[70]
	多电极	单频阻抗数据；多频阻抗数据	5, 6, 7, 10 μm珠子；红细胞；酵母细胞	[72-77]
	“一字型”压缩通道	细胞膜比电容、质电导率和细胞直径	表征数百个肿瘤细胞固有电学特征	[78]
	“十字型”压缩通道	细胞膜比电容和细胞质电导率	表征数十万个肿瘤细胞固有电学特征	[79]
	“双T型”压缩通道	细胞膜比电容、质电导率和细胞直径	表征数十万个肿瘤细胞固有生物电学特征	[80]

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参考文献(80)

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