基于氧化石墨烯与金属离子的逻辑模型设计与可控性验证

王璐慧; 王越; 钱梦瑶; 董亚非

doi:10.11999/JEIT190872

基于氧化石墨烯与金属离子的逻辑模型设计与可控性验证

doi: 10.11999/JEIT190872

王璐慧¹,
王越²,
钱梦瑶²,
董亚非^{1, 2, ,}

1.
陕西师范大学生命科学学院西安 710119
2.
陕西师范大学计算机科学学院西安 710119

基金项目: 国家自然科学基金(61572302)，陕西省自然科学基础研究计划(2020JM-298)，陕西师范大学博士研究生自由探索项目(2019TS075)

详细信息

作者简介:
王璐慧：女，1993年，博士生，研究方向为生物信息学、DNA计算、生物传感器

王越：女，1994年，硕士生，研究方向为DNA计算、合成生物学

钱梦瑶：女，1995年，硕士生，研究方向为DNA计算、生物传感器

董亚非：男，1963年，教授，研究方向为生物信息学、DNA计算、生物传感器、合成生物学

通讯作者:
董亚非　dongyf@snnu.edu.cn

中图分类号: TP301
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- 被引次数: 0
出版历程
- 收稿日期: 2019-11-04
- 修回日期: 2020-04-16
- 网络出版日期: 2020-05-12
- 刊出日期: 2020-06-22

Logical Model Design and Controllability Verification Based on Graphene Oxide and Metal Ions

1.
School of Life Science, Shaanxi Normal University, Xi’an 710119, China
2.
School of Computing Technology, Shaanxi Normal University, Xi’an 710119, China

Funds: The National Natural Science Foundation of China (61572302), The Basic Research Plan of Natural Science of Shaanxi Province(2020JM-298), The Free Exploration Project for Doctoral Students of Shaanxi Normal University (2019TS075)

摘要

摘要: 作为20世纪末诞生的新型学科，生物计算现已成为前沿科学研究的热点。与电子计算机相似，生物计算机的构建需要多种分子逻辑门，而将氧化石墨烯(GO)、重金属离子等具有生化特性的物质引入分子逻辑门的设计中，有望为研究提出新思路。此外，分子逻辑门最终应在生物实验层面上实现，这就需要对生物实验各个条件的可控性及可控范围进行研究。基于这样的想法，该文以氧化石墨烯和金属离子为基础设计了多个逻辑门，通过仿真实验、电泳实验、正交实验、荧光实验等验证可行性的同时，对实验的可控性及可控范围有进一步研究，一方面证明了所设计逻辑门可行性，另一方面也发现其有运用于实际样品检测的能力。
- 生物计算 /
- 逻辑门 /
- 可控性 /
- 氧化石墨烯 /
- 金属离子
Abstract: As a new subject born at the end of the 20th century, biological computing has become a hot spot of frontier scientific research. Similar to the electronic computer, the construction of biological computer needs a variety of molecular logic gates, and the introduction of Graphene Oxide(GO), heavy metal ion heavy metal particles and other substances with biochemical characteristics into the design of molecular logic gates is expected to put forward new ideas for research. In addition, molecular logic gates should be realized at the level of biological experiments, which requires the study of controllability and controllable range of various conditions of biological experiments. Based on this idea, several logic gates are designed based on graphene oxide and metal ions. The feasibility is verified by simulation experiment, electrophoresis experiment, orthogonal experiment, fluorescence experiment, etc. At the same time, the controllability and controllable range of the experiment are further researched. On the one hand, the feasibility of the designed logic gate is proved, on the other hand, it be found that can be applied to actual samples.
- Biological computing /
- Logic gate /
- Controllability /
- Graphene Oxide (GO) /
- Metal ions

HTML全文

图 1 YES门与AND门原理及真值表

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图 2 YES门及AND门仿真结果

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图 3 YES门PAGE电泳结果

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图 4 AND门琼脂糖电泳结果

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图 5 实验条件可控性验证及优化

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图 6 目标响应范围及线性相关性

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图 7 基于金属离子的多功能模型原理及真值表

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图 8 基于金属离子的多功能模型仿真结果

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图 9 实验条件可控性验证

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图 10 汞离子与银离子分别存在时目标响应范围及线性相关性

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图 11 基于氧化石墨烯与金属离子的3输入逻辑门原理、真值表及电泳结果

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表 1 DNA链序列

链名称	链序列(5’-3’)
Target	GACATTCATCACGCTCAATCACTACTT
H1	TAMRA - AAGTAGTGATTGAGCGTGATGAATGTC
HH1	AAGTAGTGATTGAGCGTGATGAATGTCACTACTTCAACTCGCATTCATCACGCTCAATC
HH2	TAMRA - TGATGAATGCGAGTTGAAGTAGTGACATTCATCACGCTCAATCACTACTTCAACTCGCA

下载: 导出CSV

表 2 DNA链序列

链名称	链序列 (5’–3’)
A	FAM–GTACACTGTAAAAAAAAAAAAAAACACTGTG–BHQ

下载: 导出CSV

表 3 汞、银离子正交结果

实验号	A链浓度 (A)	离子浓度 (B)	反应时间 (C)	F₀–F
1	A₁	B₁	C₁	1239.4	1042.8
2	A₁	B₂	C₃	1572.9	1806.4
3	A₁	B₃	C₂	1535.1	2010.4
4	A₂	B₁	C₃	3243.2	1090.8
5	A₂	B₂	C₂	6569.5	4773.2
6	A₂	B₃	C₁	7675.0	7103.0
7	A₃	B₁	C₂	3723.7	1201.6
8	A₃	B₂	C₁	8459.9	9090.4
9	A₃	B₃	C₃	11151.3	10298.2

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