基于DNA适配体的荧光生物传感器

董亚非; 胡文晓; 钱梦瑶; 王越

doi:10.11999/JEIT190860

基于DNA适配体的荧光生物传感器

doi: 10.11999/JEIT190860

董亚非^{1, 2, ,},
胡文晓¹,
钱梦瑶²,
王越²

1.
陕西师范大学生命科学学院西安 710119
2.
陕西师范大学计算机科学学院西安 710119

基金项目: 国家自然科学基金(61572302)，陕西省自然科学基础研究计划(2020JM-298)

详细信息

作者简介:
董亚非：男，1963年，教授，研究方向为DNA计算和生物传感器

胡文晓：女，1996年，硕士生，研究方向为DNA计算和生物传感器

钱梦瑶：女，1995年，硕士生，研究方向为DNA计算和生物传感器

王越：女，1995年，硕士生，研究方向为DNA计算和生物传感器

通讯作者:
董亚非　dongyf@snnu.edu.cn

中图分类号: TP212.3
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- 被引次数: 0
出版历程
- 收稿日期: 2019-11-01
- 修回日期: 2020-03-14
- 网络出版日期: 2020-04-03
- 刊出日期: 2020-06-22

DNA Aptamer-based Fluorescence Biosensor

1.
College of Life Science, Shaanxi Nomal University, Xi’an 710119, China
2.
School of Computer Science, Shaanxi Normal University, Xi’an 710119, China

Funds: The National Natural Science Foundation of China (61572302), The Natural Science Basic Research Plan in Shaanxi Province of China (2020JM-298)

摘要

摘要: 近年来，随着DNA纳米技术的飞速发展，基于DNA作为适配体的荧光生物传感器不断被大量学者研究和构建，以实现对靶标物质的灵敏快速检测。作为DNA纳米技术的新兴方向，基于DNA适配体的荧光生物传感器具有巨大的应用潜力。该文对近年来基于DNA适配体所构建的荧光生物传感器进行了总结。包括荧光信号的实现：荧光染料标记；非荧光染料标记。荧光检测信号的提升：酶介导的靶标循环和信号扩增策略；链置换反应介导的靶标循环和信号扩增策略；基于链置换反应和酶介导的靶标循环和信号扩增策略。在此基础上对基于DNA适配体的荧光生物传感器进行展望并提出建议。
- 生物传感器 /
- DNA适配体 /
- 荧光分析
Abstract: In recent years, with the rapid development of DNA nanotechnology, fluorescence biosensors based on DNA as aptamer are studied and constructed by a large number of scholars in order to realize the sensitive and rapid detection of target materials. As a new branch of DNA nanotechnology, fluorescence biosensors based on DNA aptamer have great application. The fluorescence biosensors based on DNA aptamers are summarized. The realization of fluorescence signal contains fluorescent dyes and non-fluorescent dye labeled. Enhancement of fluorescence signals involve enzyme-assisted, chain replacement reaction and both of all mediated target circulation and signals amplification strategy. On this basis, the fluorescence biosensor based on DNA aptamer is prospected and some suggestions are put forward.
- Biosensor /
- DNA aptamer /
- Fluorenscence analysis

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